Cloned (Comment) | Organism |
---|---|
catalytic and hinge domain | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E240A | polypeptide can not be refolded | Homo sapiens |
K110A | processing of the enzyme is blocked | Homo sapiens |
R108A | processing of the enzyme is blocked | Homo sapiens |
R111A | processing of the enzyme is blocked | Homo sapiens |
Y112F | normal enzyme processing | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00344 | - |
(7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-Ala-Arg-NH2 | pH 7.5, 37°C, mature and mutant enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
catalytic and hinge domain | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P50281 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
refolding of the purified polypeptide to active enzyme by gradient dialysis with urea gradient from 6 M decreasing to 0 M and 2-mercaptoethanol gradient from 150 mM decreasing to 0 mM in the presence of CaCl2 and ZnCl2 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-Ala-Arg-NH2 + H2O | fluorescent peptide | Homo sapiens | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |